Azam Asgari; Saeed Semnanian; Nafiseh Atapour; Amir Shojaee; Vahid Sheybani; Seyyed Javad Mirnajafi Zadeh
Volume 23, Issue 2 , March and April 2016, , Pages 290-303
Abstract
Background and purpose: Low frequency stimulation (LFS) has anticonvulsant effect. However, its mechanism of action has not been completely determined. In the present study the effect of LFS on evoked inhibitory post synaptic GABAergic currents (eIPSC) is investigated in CA1 pyramidal neurons of the ...
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Background and purpose: Low frequency stimulation (LFS) has anticonvulsant effect. However, its mechanism of action has not been completely determined. In the present study the effect of LFS on evoked inhibitory post synaptic GABAergic currents (eIPSC) is investigated in CA1 pyramidal neurons of the hippocampus in kindled rats. Materials and Methods: In this experimental study animals were kindled through electrical stimulation of amygdala. 24 hours following fully kindling achievement in 20 Wistar rats, the effect of LFS on eIPSCs was assessed in hippocampal slices. Results: Obtained results showed that application of LFS at 200 pulses and at the intensity of 1.5 threshold, increased the amplitude and decay time constant of eIPSCs in both control and kindled rats. When 200 pulses of LFS were administered with an intensity equal to threshold, only eIPSC amplitude was increased in both control and kindled groups significantly (P<0.001). Comparing the effectiveness of LFS on control and kindled groups showed that 200 pulses of LFS at the intensity of 1.5 threshold had higher effect in control than kindled group (P<0.001). Conclusion: Results of the present study showed that LFS application increased eIPSCs parameters in a pulse number and intensity dependent manner. This increment can be considered as a possible anticonvulsant mechanism of LFS.